NC State
Barycki Laboratory

UGDH

Brenna M Zimmer, Joseph J Barycki, and Melanie A Simpson. (2022) Mechanisms of coordinating hyaluronan and glycosaminoglycan production by nucleotide sugars. Am J Physiol Cell Physiol. 322(6):C1201-C1213.

Brenna M Zimmer, Joseph J Barycki, and Melanie A Simpson. (2021) Integration of Sugar Metabolism and Proteoglycan Synthesis by UDP-glucose Dehydrogenase. J Histochem Cytochem. 2021 Jan;69(1):13-23. PMID: 32749901.

Brenna M Zimmer, Michelle E. Howell, Linden Ma, Jeffry Enders, Danielle Lehman, Eva Corey, Joseph J Barycki, and Melanie A Simpson. (2021) Altered glucuronidation deregulates androgen dependent response profiles and signifies castration resistance in prostate cancer. Oncotarget 12: 1886-1902. PMCID: PMC9169848.

Holger Hengel, Célia Bosso-Lefèvre, George Grady, Emmanuelle Szenker-Ravi, Hankun Li, Sarah Pierce, Élise Lebigot, Thong-Teck Tan, Michelle Y Eio, Gunaseelan Narayanan, Kagistia Hana Utami, Monica Yau, Nader Handal, Werner Deigendesch, Reinhard Keimer, Hiyam M Marzouqa, Meral Gunay-Aygun, Michael J Muriello, Helene Verhelst, Sarah Weckhuysen, Sonal Mahida, Sakkubai Naidu, Terrence G Thomas, Jiin Ying Lim, Ee Shien Tan, Damien Haye, Michèl A A P Willemsen, Renske Oegema, Wendy G Mitchell, Tyler Mark Pierson, Marisa V Andrews, Marcia C Willing, Lance H Rodan, Tahsin Stefan Barakat, Marjon van Slegtenhorst, Ralitza H Gavrilova, Diego Martinelli, Tal Gilboa, Abdullah M Tamim, Mais O Hashem, Moeenaldeen D AlSayed, Maha M Abdulrahim, Mohammed Al-Owain, Ali Awaji, Adel A H Mahmoud, Eissa A Faqeih, Ali Al Asmari, Sulwan M Algain, Lamyaa A Jad, Hesham M Aldhalaan, Ingo Helbig, David A Koolen, Angelika Riess, Ingeborg Kraegeloh-Mann, Peter Bauer, Suleyman Gulsuner, Hannah Stamberger, Alvin Yu Jin Ng, Sha Tang, Sumanty Tohari, Boris Keren, Laura E Schultz-Rogers, Eric W Klee, Sabina Barresi, Marco Tartaglia, Hagar Mor-Shaked, Sateesh Maddirevula, Amber Begtrup, Aida Telegrafi, Rolph Pfundt, Rebecca Schüle, Brian Ciruna, Carine Bonnard, Mahmoud A Pouladi, James C Stewart, Adam Claridge-Chang, Dirk J Lefeber, Fowzan S Alkuraya, Ajay S Mathuru, Byrappa Venkatesh, Joseph J Barycki, Melanie A Simpson, Saumya S Jamuar, Ludger Schöls, Bruno Reversade (2020) Loss-of-function mutations in UDP-Glucose 6-Dehydrogenase cause recessive developmental epileptic encephalopathy. Nat Commun. 2020 Jan 30;11(1):595. PMID: 32001716.

Joseph J. Barycki. 2017. Covering their bases: The phosphobase methylation pathway in plants. Journal of Biological Chemistry. Dec 29;292(52):21703-21704. PMID 29288241.

Brenna M. Zimmer, Michelle E. Howell, Qin Wei, Linlin Ma, Trevor Romsdahl, Eileen G. Loughman, Jonathan E. Markham, Javier Seravalli, Joseph J. Barycki, and Melanie A. Simpson. 2016. Loss of exogenous androgen dependence by prostate tumor cells is associated with elevated glucuronidation potential. Hormones and Cancer. June 15, 2016. 7(4):260-71. PMID: 27307252

George Grady, Ashley Thelen, Jaleen Albers, Ju T, Jiantao Guo, Joseph J. Barycki+, Melanie A. Simpson+. 2016. Inhibiting hexamer disassembly of human UDP-glucose dehydrogenase by photoactivated amino acid cross-linking. Biochemistry. Jun 7, 2016. 55(22), 3157-64. PMID: 27198584. +co-corresponding authors

Xiaobin Wu, Heejeong Kim, Javier Seravalli, Joseph J. Barycki, P. John Hart, David Gohara, Enrico Di Cera, Won Hee Jung, Daniel J. Kosman, and Jaekwon Lee. 2016. Potassium and the K+/H+ exchanger Kha1p promote binding of copper to ApoFet3p multi-copper ferroxidase. Journal of Biological Chemistry Apr 29, 2015. 291(18), 9796-806. PMID 26966178

Hatice K. Ozer, Adrienne C. Dlouhy, Jeremy D. Thornton, Jingjing Hu, Yilin Liu, Joseph J. Barycki, Janneke Balk, and Caryn E. Outten. 2015. Cytosolic Fe-S cluster protein maturation and iron regulation are independent of the mitochondrial Erv1/Mia40 import system. Journal of Biological Chemistry Sep 22, 2015. 290(46), 27829-40. PMID 26396185

McAtee CO, Berkebile AR, Elowsky C, Fangman T, Barycki JJ, Wahl JK 3rd, Khalimonchuk O, Naslavsky N, Caplan S, Simpson MA. (2015) Hyaluronidase Hyal1 increases tumor cell proliferation and motility through accelerated vesicle trafficking. J Biol Chem. 2015 Apr 8. pii: jbc.M115.647446. [Epub ahead of print] PMID: 25855794

McAtee CO, Barycki JJ and Simpson MA. (2014) Emerging roles for hyaluronidase in cancer metastasis and therapy. Invited review. Adv Cancer Res. 2014;123:1-34. doi: 10.1016/B978-0-12-800092-2.00001-0.

Liu Y, Hyde AS, Simpson MA, Barycki JJ. (2014) Emerging regulatory paradigms in glutathione metabolism. Invited review. Adv Cancer Res. 2014;122C:69-101. doi: 10.1016/B978-0-12-420117-0.00002-5.

Hyde AS*, Thelen AM*, Barycki JJ+, Simpson MA+. (2013) UDP-glucose dehydrogenase activity and optimal downstream cellular function require dynamic reorganization at the dimer-dimer subunit interfaces. J Biol Chem 288(49): 35049-35057. *Co-first authors, +Co-coressponding authors.

Hyde AS, Farmer EL, Easley KE, van Lammeren K, Christoffels VM, Barycki JJ, Bakkers J, and Simpson MA. (2012) UDP-glucose dehydrogenase polymorphisms from patients with congenital heart valve defects disrupt enzyme stability and quaternary assembly. J Biol Chem 287(39): 32708-16.

Joseph J. Barycki. 2012. Glutathione. In Encyclopedia of Biophysics. Ed. Victor Davidson. Publisher: Springer Publishing, Inc. (Publication date March 29, 2012).

Melanie Willis, Yilin Liu, Ekaterina I. Biterova, Heejeong Kim, Melanie A. Simpson, Jaekwon Lee, and Joseph J. Barycki. 2011. Enzymatic defects underlying hereditary glutamate cysteine ligase deficiency are mitigated by association of the catalytic and regulatory subunits. Biochemistry. 50(29), 6508-17. PMID: 21657237

Ekaterina I. Biterova and Joseph J. Barycki. 2010. Structural basis for feedback and pharmacological inhibition of Saccharomyces cerevisiae glutamate cysteine ligase. Journal of Biological Chemistry. 285(19), 14459-14466.

Ekaterina I. Biterova and Joseph J. Barycki. 2009. Mechanistic details of glutathione biosynthesis revealed by crystal structures of S. cerevisiae glutamate cysteine ligase. Journal of Biological Chemistry. 284(47), 32700-32708.

Kristin Williams, Sierra Cullati, Aaron Sand, Ekaterina I. Biterova and Joseph J. Barycki. 2009. Crystal structure of acivicin-inhibited gamma-glutamyltranspeptidase reveals a critical role for the C-terminus in autoprocessing and catalysis. Biochemistry. 48(11), 2459-2467.

Ling Zhang, Alamelu G. Bharadwaj, Andrew Casper, Joel Barkley, Joseph J. Barycki and Melanie A. Simpson. 2009. Hyaluronidase activity of human Hyal1 requires active site acidic and tyrosine residues. Journal of Biological Chemistry. 282(14), 9433-9442.

Amy Morrow, Kristin Williams, Aaron Sand, Gina Boanca, and Joseph J. Barycki. 2007. Characterization of H. pylori gamma-glutamyltranspeptidase reveals the molecular basis for substrate specificity and a critical role for the tyrosine 433-containing loop in catalysis. Biochemistry, 46 (46), 13407-13414.

Joseph J. Barycki. 2007. Chapter 2: Glutathione. In Redox Biology. Ed. Ruma Banerjee. Publisher: John Wiley and Sons, Inc.

Katherine E. Easley, Brandi J. Sommer, Gina Boanca, Joseph J. Barycki, and Melanie A. Simpson. 2007. Characterization of human UDP-glucose dehydrogenase reveals critical catalytic roles for lysine 220 and aspartate 280. Biochemistry, 46(2), 369-78.

Gina Boanca, Aaron Sand, Toshihiro Okada, Hideyuki Suzuki, Hidehiko Kumagai, Keiichi Fukuyama, and Joseph J. Barycki. 2007. Autoprocessing of H. pylori gamma-glutamyltranspeptidase leads to the formation of a threonine-threonine catalytic dyad. Journal of Biological Chemistry, 282 (1), 534-541.

Gina Boanca, Aaron Sand, and Joseph J. Barycki. 2006. Uncoupling enzymatic and autoprocessing activities of H. pylori gamma-glutamyltranspeptidase. Journal of Biological Chemistry 281 (28), 19029-19037.

Ekaterina I. Biterova, Anton A. Turanov, Vadim N. Gladyshev and Joseph J. Barycki. 2005. Crystal structures of oxidized and reduced mitochondrial thioredoxin reductase provide molecular details of the reaction mechanism. Proceedings of the National Academies of Sciences 102(42), 15018-15023.

Patricia L. Herman, Mark Behrens, Sarbani Chakraborty, Brenda M. Chrastil, Joseph Barycki and Donald P. Weeks. 2005. A three component dicamba O-demethylase from Pseudomonas maltophilia, strain DI-6: Gene isolation, characterization, and heterologous expression. Journal of Biological Chemistry 280, 24759-24767.

Peter J Hanley, Stefan Dröse, Ulrich Brandt, Rachel A Lareau, Abir L Banerjee, D. K. Srivastava, Leonard J Banaszak, Joseph J Barycki, Paul P Van Veldhoven, and Jurgen Daut. 2005. 5-hydroxydecanoate is metabolised in mitochondria and creates a rate-limiting bottleneck for beta-oxidation of fatty acids. The Journal of Physiology 562(Pt 2), 307-318.

Brandi J. Sommer, Joseph J. Barycki, and Melanie A. Simpson. 2004. Characterization of human UDP-glucose dehydrogenase: Cys 276 is required for the second of two successive oxidations. Journal of Biological Chemistry 279, 23590-235906.

Joseph J. Barycki, Laurie K. O’Brien, Arnold W. Strauss, and Leonard J. Banaszak. 2001. Glutamate 170 of human L-3-hydroxyacyl-CoA dehydrogenase is required for proper orientation of the catalytic histidine and structural integrity of the enzyme. Journal of Biological Chemistry 276, 36718-36726.

Joseph J. Barycki, Laurie K. O’Brien, Arnold W. Strauss, and Leonard J. Banaszak. 2000. Sequestration of the active site by interdomain shifting. Crystallographic and spectroscopic evidence for distinct conformations of L-3-hydroxyacyl-CoA dehydrogenase. Journal of Biological Chemistry 275, 27186-27196.

Joseph J. Barycki, Laurie K. O’Brien, Jens J. Birktoft, Arnold W. Strauss, and Leonard J. Banaszak. 1999. Pig heart short chain L-3-hydroxyacyl-CoA dehydrogenase revisited: Sequence analysis and crystal structure determination. Protein Science 8(10), 2010-2018.

Joseph J. Barycki, Laurie K. O’Brien, Judy M. Bratt, Rongguang Zhang, Ruslan Sanishvili, Arnold W. Strauss, and Leonard J. Banaszak. 1999. Biochemical characterization and crystal structure determination of human heart short chain L-3-hydroxyacyl-CoA dehydrogenase provide insights into catalytic mechanism. Biochemistry 38(18), 5786-5798.

Joseph J. Barycki and Roberta F. Colman. 1997. Identification of the non-substrate binding site of rat liver glutathione S-transferase, isozyme 1-1, by the steroid affinity label, 3beta-(iodoacetoxy)dehydroisoandrosterone. Archives of Biochemistry and Biophysics 345, 16-31.

Jibo Wang, Joseph J. Barycki and Roberta F. Colman. 1996. Tyrosine 8 contributes to catalysis but is not required for activity of rat liver glutathione S-transferase, 1-1. Protein Science 5, 1032-1042.

Joseph J. Barycki and Roberta F. Colman. 1993. Affinity labeling of glutathione S-transferase, isozyme 4-4, by 4-(fluorosulfonyl)benzoic acid reveals Tyr 115 to be an important determinant of xenobiotic substrate specificity. Biochemistry 32, 13002-13011.